HIF-1α (Hypoxia-Inducible Factor-1α) Ab-4, Mouse Monoclonal Antibody,Lab Vision™ Epredia™

HIF-1 (hypoxia-inducible factor-1) is a heterodimer consisting of an alpha and a β subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF-1 is a key component of a widely operative transcriptional response activated by hypoxia, cobaltous ions, and iron chelation. HIF-1 activates transcription of hypoxia-inducible genes, including those encoding: erythropoietin, vascular endothelial growth (VEGF), heme oxygenase-1, inducible nitric oxide synthase, and the glycolytic enzymes aldolase A, enolase 1, lactate dehydrogenase A, phosphofructokinase I, and phosphoglycerate kinase 1. The C-terminal of HIF-1 alpha binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF.

Host Species: Mouse

Clone: H1α67

Isotype: IgG2b/κ

Species Reactivity: Human, Sheep, Mouse, Rat and Ferret. Others not known.

Epitope: aa 432-528

Immunogen: Recombinant hHIF-1α (aa 432-528) protein

Molecular Weight: 120kDa

Positive Control: Mammalian cells cultured under reduced O₂ tension

Cellular Localization: Nuclear

Recommended for:

• Immunohistochemistry (Formalin/paraffin)
• Western Blotting (Not verified)
• Immunoprecipitation (Not verified)

Note:

USA: RUO; Int'l: RUO