Invitrogen™ IL-10RA (CD210a) Human ELISA Kit

Beschreibung

The Human Interleukin 10 Receptor Alpha (CD210a) (Hu IL-10RA) ELISA quantitates Hu IL-10RA in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-10RA. Principle of the method The Human IL-10RA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Das Protein, das von diesem Gen codiert wird, ist ein Rezeptor für Interleukin 10. Dieses Protein ist strukturell mit Interferon-Rezeptoren verwandt. Es vermittelt nachweislich das immunsuppressive Signal von Interleukin 10 und hemmt dadurch die Synthese proinflammatorischer Zytokine. Dieser Rezeptor fördert das Überleben von myeloischen Vorläuferzellen über den Insulin-Rezeptor-Substrat-2/PI3-Kinase/AKT-Signalweg. Die Aktivierung dieses Rezeptors führt zur Tyrosinphosphorylation von JAK1- und TYK2-Kinasen. Für dieses Gen wurden zwei Transkriptvarianten gefunden, jedoch codiert nur eine Variante Proteine.