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PeproTech IL-4 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Beschreibung
AA Sequence of recombinant protein: MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-4. Anti-Human IL-4-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hIL-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-4 (500-P24) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIL-4. Western Blot: To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P24BT-1MG will be provided as 2 x 500 μg
Spezifikation
Spezifikation
| Antigen | IL-4 |
| Anwendungen | ELISA, Western Blot |
| Klassifikation | Polyclonal |
| Konzentration | 0.1-1.0 mg/mL |
| Konjugat | Biotin |
| Zusammensetzung | PBS with no preservative |
| Gen | Il4 |
| Gen-Zugriffsnummer | P05112 |
| Gen-Alias | B cell growth factor 1; B_cell stimulatory factor 1; B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; cytokine; H-IL-4; IGG1 induction factor; IL2; IL4; Il-4; Il4e12; ILN; Interleukin; interleukin 4; interleukin 4 variant 2; interleukin 4 variant IL-4delta2; interleukin 4 variant IL-4delta3; interleukin 4 variant IL-4int2A; interleukin 4 variant IL-4int3A; interleukin 4 variant IL-4int3B; Interleukin4; interleukin-4; interleukin-4 precursor; lymphocyte stimulatory factor 1; MGC79402; M-IL-4; Pitrakinra |
| Gensymbole | Il4 |
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