Thermo Scientific™ CaptureSelect™ LambdaXP MiniChrom™ Pre-packed Column

These pre-packed columns are ready to connect and have the following features:

• Dimensions of 1-mL column: 0.8 x 2 cm
• Dimensions of 5-mL column: 0.8 x 10 cm
• Direct connection to standard chromatography systems
• Packed with CaptureSelect LambdaXP Affinity Matrix, offering high dynamic binding capacity

The 1-mL columns can be used for quick screenings of application feasibility and lab-scale purification on a convenient and easy-to-use pre-packed column. The 10-cm bed height of the 5-mL columns allows initial process development on a bench scale.

CaptureSelect LambdaXP affinity matrix recognizes the constant domain of the human lambda light chain enabling purification of all IgG isotypes including IgG, IgA, IgM, IgD, and IgE directly from complex source materials in a robust and efficient purification process with excellent purity obtained in a single step.

Features of the LambdaXP affinity matrix include:

• Efficient elution of Fab fragments and immunoglobulins containing a lambda light chain, no cross binding to the Fc-region or kappa light chains
• One-step purification from crude material with high purity and yield
• Human specific
• Non–animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

• Matrix: agarose-based, epoxide-activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect LambdaXP affinity ligand
• Ligand coupling: epoxide coupling
• Binding capacity: >30 g IgG/L resin depending on flow rate, column height, and contact time
• Elution conditions: 25 mM sodium acetate, pH 3.5
• Flow characteristics: 50–150 cm/h (up to 2 bar)
• Formulation buffer: 20% (v/v) ethanol