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Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Beschreibung
Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.
Features of Lyo-ready RPA products
- Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
- Robust and specific RNA/DNA amplification
- Amplification from inhibitor-containing samples
- Fast target amplification in as little as 20 minutes
- Ability to easily optimize RPA or RT-RPA reactions
Kit components
The Lyo-ready RPA Kit includes all required components for an RPA reaction:
- Lyo-ready T4 UvsX Protein—produced from E. coli cells bearing cloned uvsX gene derived from bacteriophage T4
- Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
- Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
- Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced from E. coli cells carrying cloned bacteriophage T4 gene 32
- Optimized reaction buffer, magnesium chloride, and dNTPs
Spezifikation
Spezifikation
| Produkttyp | Lyo-bereites RPA-Kit |
| Form | Flüssig |
| Zur Verwendung mit (Anwendung) | RPA, RT-RPA |
| Polymerase | Lyo-ready Bst DNA Polymerase |
| Menge | 100 Reaktionen |
| Forschungskategorie | Nucleic Acid Amplification |
| Inhalt und Lagerung | • Lyo-ready T4 UvsX Protein (1.2 mg/mL), 50 μL |
| Optimale Reaktionstemperatur | 42 °C |
| Reverse Transkriptase | SuperScript IV Reverse Transcriptase |
| Versandbedingung | Dry Ice |
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Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
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