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Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Artikelnummer. 30245214
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Menge:
100 Reaktionen
120 μg
1600 μg
2000 μl
Produkttyp:
Lyo-bereites RPA-Kit
Lyo-bereites T4-Gen-32-Protein
Lyo-bereites T4-UvsX-Protein
Lyo-ready T4 UvsY Protein
Reaktionspuffer
Unit Size:
100 Reaktionen
120 Mikrogramm
1600 Mikrogramm
2000 Mikroliter
Product Code. Menge Produkttyp unitSize
30245214 120 μg Lyo-bereites T4-UvsX-Protein 120 Mikrogramm
30245335 1600 μg Lyo-bereites T4-Gen-32-Protein 1600 Mikrogramm
30245152 120 μg Lyo-ready T4 UvsY Protein 120 Mikrogramm
30245027 2000 μl Reaktionspuffer 2000 Mikroliter
30245142 100 Reaktionen Lyo-bereites RPA-Kit 100 Reaktionen
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Product Code. 30245214

Brand: Invitrogen™ A72124

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Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.

Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.

Features of Lyo-ready RPA products

  • Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
  • Robust and specific RNA/DNA amplification
  • Amplification from inhibitor-containing samples
  • Fast target amplification in as little as 20 minutes
  • Ability to easily optimize RPA or RT-RPA reactions

Kit components

The Lyo-ready RPA Kit includes all required components for an RPA reaction:

  • Lyo-ready T4 UvsX Protein—produced from E. coli cells bearing cloned uvsX gene derived from bacteriophage T4
  • Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
  • Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
  • Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced from E. coli cells carrying cloned bacteriophage T4 gene 32
  • Optimized reaction buffer, magnesium chloride, and dNTPs
TRUSTED_SUSTAINABILITY

Specifications

Produkttyp Lyo-bereites T4-UvsX-Protein
Form Flüssig
Zur Verwendung mit (Anwendung) RPA, RT-RPA
Menge 120 μg
Forschungskategorie Nucleic Acid Amplification
Inhalt und Lagerung Lyo-ready T4 UvsX Protein, 60 μL, Store at -25°C to -15°C.
Optimale Reaktionstemperatur 42 °C
Versandbedingung Dry Ice
Konzentration 2 mg/mL
Reaktionszeit Up to 20 min.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

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