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Invitrogen™ MembranePro™ Functional Protein Support Kit
Description
Benefits of MembranePro™ include:
- Proteins are displayed on human (or other mammalian) cell membranes
- Cellular quality control and mammalian posttranslational processing helps produce functional protein
- Proteins bud off as lipoparticles and are easily collected from the culture medium
- Lipoparticles are enriched with expressed receptors
MembranePro™ workflow is less labor intensive in comparison to traditional production of membrane fractions. Briefly, the MembranePro™ protocol allows you to:
- Clone your gene of interest using a TOPO™ vector
- Transfect with Lipofectamine™ 2000 and MembranePro™ Reagent into 293FT cells
- Harvest lipoparticles 48h post-transfection, concentrate with MembranePro™ Precipitation Mix
- Store lipoparticles at -80 C or proceed to downstream assays
MembranePro™ products are offered in two configurations – as an expression kit (TOPO™ vector, Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent, 293FT cells) and as a support kit (Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent). The expression kit is only available in a 10 reaction size; the support kit is available in 10, 60 and 600 reaction sizes.
Specifications
Specifications
| Type | MembranePro Functional Protein Support Kit |
| Sufficient For | 10 Reactions |
| Delivery Type | Virus-Like Particles (VLPs) |
| Quantity | 10 reactions kit |
| Content And Storage | •1 x 75 ml MembranePro™ Precipitation Mix (Store at room temperature) •1 x 0.3 mg (1 mg⁄ml) MembranePro™ Reagent (Store at -20°C) •3 x 0.75 ml Lipofectamine™ 2000 (Store at +4°C) •Manual |
| Selection Agent (Eukaryotic) | None |
| Expression Mechanism | Cell-Based Expression |
| High-throughput Compatibility | High-throughput Compatible |
| Expression System | Mammalian |
| Expressed Protein Type | Membrane Protein |
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For Research Use Only. Not for use in diagnostic procedures.
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