Invitrogen™ Mouse anti-Rat IgG2a Secondary Antibody, eFluor™ 570, eBioscience™

Mouse Monoclonal Secondary Antibody

Description: The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2a or any mouse isotype antibodies. Applications Reported: This R2a-21B2 antibody has been reported for use in immunocytochemistry, and immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections. Applications Tested: This R2a-21B2 antibody has been tested by immunocytochemistry on fixed and permeabilized cells that have been stained first with a rat IgG2a anti-mouse antibody. This can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable. Excitation: 555 nm; Emission: 570 nm.