Invitrogen™ MULT1 (NKG2D Ligand) Monoclonal Antibody (5D10), eBioscience™

Armenian Hamster Monoclonal Antibody

Description: The 5D10 monoclonal antibody reacts with the murine MULT-1 (Murine ULBP-Like Transcript 1). MULT-1 is a type I transmembrane protein with two Ig-like domain and is one of the known murine NKG2-D ligands that include RAE1 molecules (alpha, beta, epsilon, gamma and delta) and H60. MULT-1 is a high affinity ligand for NKG2D.Expression of NKG2-D ligands is low or absent on normal adult tissues. However, stressed or transformed cells express NKG2-D ligand and can activate NK cell tumoricidal activity. Until now, the expression of NKG2-D ligands has been mainly studied with NKG2-D tetramers recognizing all NKG2-D ligands. Applications Reported: This 5D10 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. Applications Tested: This 5D10 antibody has been tested by flow cytometric analysis of the A20 cell line. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.