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Lonza™ MycoAlert™ Mycoplasma Detection Kit (10 Tests)
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Menge:
2 x 600 μl MycoAlert-Reagens, 2 x 600 μl MycoAlert-Substrat, 1 x 10 ml MycoAlert-Assaypuffer
Packungsgröße:
10 Tests
Beschreibung
- Convenient and fast.
- Simply add two reagents to the culture supernatant.
- Perform two luminescence readings.
- Results in less than 20 min.
- Easy interpretation of results .
- Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma).
- Negligible interference with media components.
- Selective biochemical test that exploits the activity of mycoplasmal enzymes that are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells.
- Viable mycoplasma in a test sample (cell supernatant) are lysed and the enzymes react with the MycoAlert™ Substrate, catalyzing the conversion of ADP to ATP.
- The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlert™ Reagent.
- By measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlert™ Substrate (read B), a ratio can be obtained which is indicative of the presence or absence of mycoplasma.
Spezifikation
Spezifikation
| Farbe | Light yellow |
| Inhalt und Lagerung | MycoAlert Reagent (lyophilized), MycoAlert Substrate (lyophilized), MycoAlert Assay Buffer |
| Zur Verwendung mit (Anwendung) | Mykoplasmennachweis |
| Menge | 2 x 600 μl MycoAlert-Reagens, 2 x 600 μl MycoAlert-Substrat, 1 x 10 ml MycoAlert-Assaypuffer |
Sicherheit und Handhabung
Gefahrenhinweis
- EUH210-Sicherheitsdatenblatt auf Anfrage erhältlich.
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