Invitrogen™ ELISA-Kit, human, für PRAS40 (Phospho) [pT246]

Beschreibung

The Human PRAS40 (Phospho) [pT246] ELISA Kit is designed to detect and quantify the level of PRAS40 (Phospho) [pT246] in fresh or frozen human cell lysates. The assay recognizes both natural and recombinant human PRAS40 (Phospho) [pT246]. Principle of the method A monoclonal capture antibody specific for PRAS40 (Phospho) [pT246] has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Der AKT-Signalweg trägt zur Regulation der Apoptose nach verschiedenen Zelltodsignalen bei. AKT1S1, auch als PRAS40 bezeichnet, ist ein Prolin-reiches Substrat der AKT1-Kinase und spielt vermutlich eine Rolle in der Neuroprotektion, die durch den Nervenwachstumsfaktor (NGF) nach transienter fokaler zerebraler Ischämie vermittelt wird. AKT1S1 ist auch ein Substrat und potentieller Regulator des Säugetierziels von Rapamycin (mTOR), einer Serin/Threonin-Kinase, die das Zellwachstum und den Zellzyklus reguliert, und ein Negativregulator der Autophagie. Die Behandlung mit dem insulinähnlichen Wachstumsfaktor (1IGF1) kann die Phosphorylierung von AKT1S1 über den PI3K/AKT-Signalweg in PC12-Zellen induzieren.

Bestellinformation

Versandbedingung: Nasseis