Invitrogen™ NuPAGE™ Tris-Acetate Midi Protein Gels, 3 to 8%, 1.0 mm, WedgeWell™ format

Invitrogen NuPAGE Tris-Acetate WedgeWell format protein gels provide excellent separation of large molecular weight proteins. They are high performance polyacrylamide gels that simulate the denaturing or native conditions of the traditional Laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of high molecular weight proteins compared to traditional Tris-glycine SDS-PAGE gels.

Product features

• High resolution—gels offer optimal separation of high molecular weight proteins
• Better protein integrity—sample preparation process has been optimized to help preserve proteins
• Longer shelf life—gels can be stored for nine months at room temperature
• Wedge-shaped wells—for easier sample loading and up to two times higher sample volume

Run your proteins in native or denatured form

NuPAGE Tris-Acetate protein gels do not contain SDS and can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using NuPAGE LDS Sample Buffer (NP0007) and NuPAGE Tris-Acetate SDS Running Buffer (LA0041). For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer (LC2673) and Novex Tris-Glycine Native Running Buffer (LC2672).

NuPAGE WedgeWell Tris-Acetate Midi gels can be run in the SureLock Tandem Midi Gel Tank (STM1001) or XCell4 SureLock Midi-Cell (WR0100).

For transfer of proteins to a membrane, rapid semi-dry transfer can be done using the Power Blotter or rapid dry transfer using the iBlot 3 Gel Transfer Device (IB31001). Alternatively, traditional wet transfer can be performed using the SureLock Tandem Midi Blot Module (STM2001) using NuPAGE Transfer buffer (NP00061).

Convenient to use

NuPAGE Tris-Acetate gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.