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O-GlcNAc Monoclonal Antibody (RL2), Alexa Fluor™ 647, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Marke:  Affymetrix eBioscience 51-9793-41

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Artikelnummer. 16300974

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Beschreibung

Beschreibung

Description: This RL2 monoclonal antibody recognizes proteins with O-N-acetylglucosamine (O-GlcNAc) glycosylation. It was originally developed by immunizing mice with the rat liver nuclear envelopes containing nuclear pore complexes. The RL2 clone has been successfully used in Western blot, immunofluorescence, immunoprecipitation, and flow cytometry in a wide variety of mammalian cells. Applications Reported: This RL2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This RL2 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This may be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser

O-GlcNAc Monoclonal antibody specifically detects O-GlcNAc in All, Human samples. It is validated for Flow Cytometry
TRUSTED_SUSTAINABILITY
Spezifikation

Spezifikation

O-GlcNAc
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
O15294
OGT
Rat liver nuclear envelopes
25 Tests
Primary
All species, Human
Antibody
IgG1 κ
Flow Cytometry
RL2
Alexa Fluor 647
OGT
1110038P24Rik; 4831420N21Rik; AI115525; GlcNAc; HINCUT-1; HRNT1; O-GLCNAC; O-GlcNAc transferase subunit p110; Ogt; Ogtl; O-linked N-acetylglucosamine transferase 110 kDa subunit; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
Mouse
Affinity chromatography
RUO
8473
4° C, store in dark, DO NOT FREEZE!
Liquid
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