missing translation for 'onlineSavingsMsg'
Learn More
Learn More
-peptide-bound-to-H-2Kb-Flow-20170216130641.jpg-650.jpg)
Invitrogen™ OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), PE-Cyanine7, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Marke: Invitrogen™ 25-5743-82
Dieser Artikel kann nicht zurückgegeben werden.
Rückgaberichtlinie anzeigen
Beschreibung
Description: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide. This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo. Applications Reported: This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio25-D1.16 (25-D1.16) antibody has been tested by flow cytometric analysis of SIINFEKL-pulsed C57Bl/6 splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal antibody specifically detects OVA257-264 (SIINFEKL) peptide bound to H-2Kb in Mouse samples. It is validated for Flow Cytometry.
Spezifikation
| OVA257-264 (SIINFEKL) peptide bound to H-2Kb | |
| Monoclonal | |
| 0.2 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| Mouse | |
| 100 μg | |
| Primary | |
| 4°C, store in dark, DO NOT FREEZE! | |
| Liquid |
| Flow Cytometry | |
| eBio25-D1.16 (25-D1.16) | |
| PE-Cyanine7 | |
| H-2Kb-SIINFEKL; OVA257-264; OVA257-264 H-2Kb; OVA-Kb | |
| Affinity chromatography | |
| RUO | |
| Mouse | |
| Antibody | |
| IgG1 κ |
Berichtigung von Produktinhalten
Bitte geben Sie uns Ihr Feedback zu den Produktinhalten, indem Sie das folgende Formular ausfüllen.
Name des Produkts
For Research Use Only.
Haben Sie Verbesserungsvorschläge?Übermitteln Sie eine inhaltliche Korrektur