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Description
The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.
Enthält:
5X Phusion HF-Puffer, 50 mM MgCl2-Lösung, 100 % DMSO.
Specifications
Specifications
| Chemischer Name oder Material | HF Buffer Pack |
| Farbe | Colorless |
| Beschreibung | Phusion™ HF-Pufferpaket |
| Zur Verwendung mit (Anwendung) | Standard-PCR |
| Menge | 6 ml |
| Enthält | Das Phusion HF-Puffer-Pack (F-518L) enthält: • 5X Phusion HF-Puffer (1X Puffer liefert 1,5 mM MgCl2) • 50 mM MgCl2 • DMSO Bei -20 °C lagern. |
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
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