PeproTech PROK1 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Goat Polyclonal Antibody

AA Sequence of recombinant protein: AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human EG-VEGF. Anti-Human EG-VEGF-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hEG-VEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human EG-VEGF (500-P188G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hEG-VEGF. Western Blot: To detect Human EG-VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human EG-VEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P188GBT-1MG will be provided as 2 x 500 μg