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Invitrogen™ Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody, FITC
Rabbit Polyclonal Secondary Antibody
Marke: Invitrogen™ 31559
Beschreibung
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31559 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 31559 reacts with the light chains of mouse IgG and with those common to most mouse immunoglobulins, but does not react with the Fc portion of mouse IgG. This antibody does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Fluorescein Amax= 492 nm; Emax= 520 nm. Fluorophore/Protein: 8.9 μg/mL ~ 3 moles FITC per mole IgG (lot-dependent). Reconstitute with 1.1 mL of distilled water (1.5 mg/mL after restoration). Country of Origin: USA.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Spezifikation
| Mouse IgG F(ab')2 | |
| Polyclonal | |
| FITC | |
| Rabbit | |
| 1.5 mg | |
| Secondary | |
| 4°C, store in dark | |
| Lyophilized |
| Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry | |
| 1.5 mg/mL | |
| PBS with 15mg/mL BSA and 0.05% sodium azide; pH 7.6 | |
| Antigen affinity chromatography | |
| RUO | |
| Mouse | |
| Secondary Antibody | |
| IgG |
Safety and Handling
- Rabbit anti-Mouse IgG F(ab')2 Secondary Antibody, FITC
Signal Word
- Achtung
Hazard Category
- Akute Toxizität Kategorie 4
- LANGFRISTIG GEWÄSSERGEFÄHRDEND Chronisch 3
Hazard Statement
- H302-Gesundheitsschädlich bei Verschlucken.
- H412-Schädlich für Wasserorganismen, mit langfristiger Wirkung.
Precautionary Statement
- P264-Nach Gebrauch gründlich waschen.
- P270-Bei Gebrauch nicht essen, trinken oder rauchen.
- P273-Freisetzung in die Umwelt vermeiden.
- P301+P310-BEI VERSCHLUCKEN: Sofort GIFTINFORMATIONSZENTRUM/Arzt anrufen/
- P330-Mund ausspülen.
- P501b-Inhalt/Behälter gemäß den örtlichen/regionalen/nationalen/internationalen Vorschriften zuführen.
Supplemental information
- MIXTURE LIST-Enthält: sodium azide
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