Invitrogen™ Rat anti-Mouse IgG1 Secondary Antibody, eFluor™ 570, eBioscience™, Invitrogen™

Descripción

Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies. Applications Reported: This M1-14D12 antibody has been reported for use in immunohistochemical staining of frozen or formalin-fixed paraffin embedded tissue sections and immunocytochemistry. Applications Tested: This M1-14D12 antibody has been tested by immunocytochemistry after staining with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor™ 555 or TRITC filter is acceptable. Excitation: 555 nm; Emission: 570 nm

Mouse IgG1 Monoclonal antibody specifically detects Mouse IgG1 in Mouse samples. It is validated for Immunocytochemistry, Immunohistochemistry (Frozen).