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Invitrogen™ Rat anti-Mouse IgG2a Secondary Antibody, eFluor™ 570, eBioscience™
Beschreibung
Description: The monoclonal antibody m2a-15F8 recognizes Mouse IgG2a antibodies and can be used as a second step reagent in microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG2a or any rat isotype antibodies. Applications Reported: This m2a-15F8 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunohistochemical staining, and immunocytochemistry. Applications Tested: This m2a-15F8 antibody has been tested immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG2a isotype. This can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable. Excitation: 555 nm; Emission: 570 nm.
Spezifikation
Spezifikation
| Antigen | Mouse IgG2a |
| Anwendungen | Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunocytochemistry |
| Klassifikation | Monoclonal |
| Klon | m2a-15F8 |
| Konzentration | 0.2 mg/mL |
| Konjugat | eFluor 570 |
| Zusammensetzung | PBS with 0.09% sodium azide; pH 7.2 |
| Wirtsspezies | Rat |
| Reinigungsverfahren | Affinity chromatography |
| Menge | 25 μg |
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