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Rat kidney tissue lysate, Non-denatured; Abnova

Artikelnummer. 16099595
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Menge:
200 μg
Unit Size:
200 Mikrogramm
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Product Code. Menge Unit Size
16099595 200 μg 200 Mikrogramm
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Product Code. 16099595 Supplier Abnova Supplier No. L039W2.200ug
 Eingeschränkter Artikel
Verfügbar ab: 29-09-2026
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Description

Western Blotting, Immunoprecipitation

Specifications

Zur Verwendung mit (Anwendung) Immunoprecipitation, Western Blot
Tissue Kidney
Beschreibung Whole tissue lysate (non-denatured)
Lysepuffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Vorbereitungsmethode Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
Qualitätskontrollen 12.5% SDS-PAGE Stained with Coomassie Blue.
Menge 200 μg
Lagerungspuffer In modified RIPA Lysis Buffer.
Wirtsspezies Rat
Konzentration 5 mg/mL
Inhalt und Lagerung Lagerung bei -80 °C. Aliquot, um wiederholtes Einfrieren und Autauen zu vermeiden.
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