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R&D Systems™ Recombinant Human TNF-alpha Protein
Beschreibung
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL. The specific activity of Recombinant Human TNF-alpha is approximately 7.6 x 104 IU/μg, which is calibrated against human TNF-alpha WHO International Standard (NIBSC code: 88/786). Specific activity is for reference purposes only and is not routinely tested.
Spezifikation
Spezifikation
| Zusammensetzung | Lyophilized from a 0.2μm filtered solution in PBS. |
| Gen-ID (Entrez) | 7124 |
| Molekulargewicht | 17.5 kDa |
| Name | TNF-alpha |
| Menge | 5 μg |
| Quelle | E. coli-derived human TNF-alpha protein Val77-Leu233, with and without an N-terminal Met |
| Endotoxin-Konzentration | Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL. |
| Gen-Alias | APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha |
| Konjugat | Unconjugated |
| Rekombinant | Recombinant |
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