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R&D Systems™ Recombinant Human Ubiquitin Mutant (No Lysine) Protein
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1mg; Carrier Free
Beschreibung
Recombinant Human Ubiquitin Mutant (No Lysine) can be conjugated to substrate proteins via the subsequent actions of a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Recombinant Human Ubiquitin Mutant (No Lysine) is unable to form chains, making it ideal for use as a negative control for chain formation or to confirm multi-mono-ubiquitination of a substrate. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin Mutant (No Lysine) concentration of 0.2-1 mM.
Spezifikation
Spezifikation
| Zugriffsnummer | P0CG47.1 |
| Zusammensetzung | Lyophilized from a 0.2 μm filtered solution in deionized water. |
| Gen-ID (Entrez) | 7314 |
| Molekulargewicht | 8.8 kDa |
| Name | Ubiquitin Mutant No K |
| Menge | 1mg |
| Quelle | E. coli-derived human Ubiquitin protein Met1 - Gly76 Contains Lys to Arg substitutions at the following positions: 6, 11, 27, 29, 33, 48, 63 |
| Lagerungsbedingungen | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after reconstitution. |
| Konjugat | Unconjugated |
| Rekombinant | Recombinant |
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