Invitrogen™ TCR V beta 13.2 Monoclonal Antibody (H132), PE, eBioscience™

Mouse Monoclonal Antibody

Description: This H132 monoclonal antibody recognizes the human T cell receptor (TCR) Vbeta13.2 allele. Composed of an alpha and beta chain, TCR specificity is typically determined by Valpha, Jalpha, Vbeta, Dbeta, and Jbeta gene rearrangement. Vbeta expression in humans has been examined in studies on the effects of superantigens, inflammation, autoimmune disease, and HIV infection. More recently, assessment of TCR Vbeta expression has been used to phenotype T cell clonality in CD3+/TCRalpha beta+ large granular lymphocyte leukemias. A member of the Ig superfamily, this receptor is expressed on a subset of peripheral blood T cells. The H132 monoclonal antibody recognizes TCR Vbeta13.2 specifically, but not Vbeta13.1 or Vbeta13.3. Applications Reported: This H132 antibody has been reported for use in flow cytometric analysis. Applications Tested: This H132 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.