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TRA-1-60 (Podocalyxin) Mouse anti-Human, Clone: TRA-1-60, eBioscience™

Mouse Monoclonal Antibody

Marke:  Affymetrix eBioscience 14-8863-82

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Artikelnummer. 15258107

  • 232.00 € / 100 Mikrogramm

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Beschreibung

Beschreibung

Description: The TRA-1-60 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation. Contrary to early reports that the TRA-1-60 epitope can be destroyed by neuraminidase digestion, new strudies have shown that TRA-1-60 recognizes a neurominidase resistent antigen. The TRA-1-60 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer. Applications Reported: This TRA-1-60 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting, and immunofluorescence. Applications Tested: This TRA-1-60 antibody has been tested by flow cytometric analysis of the human embryonal carcinoma (EC) line 2102Ep. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This antibody has also been tested by immunoblotting of 2102Ep cell extracts, yielding a band of approximately 200-250 kDa (reducing and non-reducing conditions). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
Spezifikation

Spezifikation

TRA-1-60 (podocalyxin)
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Keratan sulfate antigen TRA1-60; TRA160
IgM
100 μg
4° C
Primary
Human
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
TRA-1-60
Unconjugated
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

For Research Use Only.