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Invitrogen™ Human VEGF-165 Recombinant Protein
Beschreibung
Mature human VEGF165 was expressed in Pichia pastoris, KM71. GenBank Accession # 003376, amino acids 27 - 191. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: EGGGQNHHEV. Purity is >95% by SDS-PAGE analyses. Activity: The biological activity of recombinant human VEGF165 was determined by measuring the proliferation of human umbilical vein endothelial cells (HUVEC). Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments. Reconstitution and Dilution: Store lyophilized material at 4°C. Reconstitute in 1 mL of distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70°C, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.
Spezifikation
Spezifikation
| Zugriffsnummer | P15692 |
| Zur Verwendung mit (Anwendung) | Bioactivity |
| Zusammensetzung | PBS with no preservative |
| Gen-ID (Entrez) | 7422 |
| Molekulargewicht | 19.2 kDa |
| Name | Human VEGF-165 |
| Reinigungsverfahren | Purified |
| Menge | 20 μg |
| Quelle | Yeast |
| Lagerungsbedingungen | 4°C |
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