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Invitrogen™ Villin Monoclonal Antibody (SP145)
Rabbit Monoclonal Antibody
Marke: Invitrogen™ MA516408
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Beschreibung
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is colon, small intestine or kidney tissue.
Villin is part of cytoskeleton associated with the microvillar actin core bundle of gastrointestinal and renal brush border. It can interact with actin in a Ca2+ and phosphoinositide-regulated manner. Villin is a very specific marker for adenocarcinomas of the gastrointestinal tract and the pancreas. It is also expressed in some Merkel cell tumor and adenocarcinoma of the lung, prostate, ovarian and kidney.
Spezifikation
| Villin | |
| Monoclonal | |
| 0.107 mg/mL | |
| PBS with 1% BSA and 0.1% sodium azide; pH 7.6 | |
| P09327, Q62468 | |
| VIL1 | |
| Synthetic peptide derived from the C-terminus of human villin protein. | |
| 500 μL | |
| Primary | |
| Human, Mouse, Rat | |
| Antibody | |
| IgG |
| Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry | |
| SP145 | |
| Unconjugated | |
| VIL1 | |
| D2S1471; OTTHUMP00000207159; Vil; Vil1; villin 1; villin-1 | |
| Rabbit | |
| Protein A/G | |
| RUO | |
| 22349, 7429 | |
| 4°C, do not freeze | |
| Liquid |
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