Protein Biology

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled

Cytiva (Formerly GE Healthcare Life Sciences) Disposable PD-10 Desalting Columns

Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. Cytiva Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.

Thermo Scientific™ DNA Gel Loading Dye (6X)

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.

Thermo Scientific™ Pierce™ LTQ Velos ESI Positive Ion Calibration Solution

Calibrate LTQ Velos, LTQ Orbitrap and other mass spectrometers using a mixture of highly purified ionizable molecules designed for positive mode calibration.

Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)

Ready-to-use reagent to reduce protein samples for protein gel electrophoresis

Invitrogen™ NuPAGE™ Antioxidant

Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Invitrogen™ SimplyBlue™ SafeStain

Less hazardous and does not require methanol or acetic acid fixatives or destains

Thermo Scientific™ Pierce™ 16% Formaldehyde (w/v), Methanol-free

Vials of high-quality, methanol-free formaldehyde, a general IHC fixative and reversible amine-crosslinking agent for protein and nucleic acid samples.

Thermo Scientific™ RIPA Lysis and Extraction Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Thermo Scientific™ Zeba™ Spin Desalting Columns, 7K MWCO, 0.5 mL

Effectively remove salt and other molecules (7K MWCO) from samples and attain excellent protein recovery with these centrifuge-based chromatography columns.

Invitrogen™ NuPAGE™ Transfer Buffer (20X)

Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting

Thermo Scientific™ Pierce™ Negative Ion Calibration Solution

Calibrate LTQ mass spectrometer instruments using a mixture of highly purified ionizable molecules designed for negative mode calibration.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Thermo Scientific™ 1-Step™ Ultra TMB-ELISA Substrate Solution

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

Invitrogen™ Colloidal Blue Staining Kit

Five times more sensitivity than Coomassie™ R-250— and can detect as little as 10ng of BSA

Thermo Scientific™ Pierce™ LAL Chromogenic Endotoxin Quantitation Kit

Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay.

Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)

Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels

Molecular Probes™ Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)

Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)

Thermo Scientific™ Pierce™ HeLa Protein Digest Standard

Use this standard, a highly validated mammalian protein digest, as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

Invitrogen™ iBlot™ Transfer Stack, nitrocellulose, mini

iBlot Transfer Stacks are used to transfer proteins

Cytiva (Formerly GE Healthcare Life Sciences) Streptavidin Sepharose™ High Performance Medium

Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium.

Thermo Scientific™ Orange DNA Loading Dye (6X)

Prepare DNA markers and samples for loading on agarose or polyacrylamide gels withThermo Scientific™ 6X Orange Loading Dye.

Invitrogen™ Magnetic Stand-96

Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories

Spectrum™ Trial Size Kits for Biotech-Grade CE Dialysis Membrane Tubing (1 meter), MWCO: 300 kd

Ideal for small, critical dialysis applications that require the more concise selectivity and retention provided by Biotech Cellulose Ester (CE) Membrane than Standard Grade RC

Thermo Scientific™ Pierce™ LTQ ESI Positive Ion Calibration Solution

Calibrate LTQ, LTQ Orbitrap, LXQ and LCQ FLEET mass spectrometers using a mixture of highly purified ionizable molecules designed for positive mode calibration.

Cytiva (Formerly GE Healthcare Life Sciences) PD SpinTrap™ G-25 Desalting Columns

Use for convenient and fast small-scale cleanup of proteins and other large biomolecules (>5000 Mr).  Cytiva Life Sciences™ PD SpinTrap™ G-25 Desalting Columns provide the option of running multiple samples in parallel.

Cytiva (Formerly GE Healthcare Life Sciences) Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

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