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ACROBiosystems Mouse CD73 / NT5E Protein, His Tag (active enzyme)
Marke: ACROBiosystems CD3-M52H9-100ug
Weitere Details : Netto-Gewicht : 0.00010kg
Beschreibung
5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 59.9 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.Spezifikation
NP_035981 | |
100 μg | |
Trp 29 - Ser 551 | |
CD73,NT5E,5 '-Nucleotidase,5 '-NT,NT5,NTE | |
Unconjugated | |
His Tag | |
Less than 1.0 EU / μg by the LAL method. | |
Powder | |
>90% as determined by SDS-PAGE |
59.9 kDa | |
Mouse CD73, His Tag (CD3-M52H9) is expressed from human 293 cells (HEK293). It contains AA Trp 29 - Ser 551 (Accession # Q61503-1). | |
-20°C | |
CD73 | |
Mouse | |
20 mM Tris, 120 mM NaCl, pH7.5 | |
HEK293 | |
Enzymes |