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ACROBiosystems ActiveMax™ Human LIF Protein, Tag Free
Marke: ACROBiosystems LIF-H521B-20UG
Weitere Details : Netto-Gewicht : 0.00001kg
Beschreibung
Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.
This protein carries no "e;tag"e;. The protein has a calculated MW of 19.9 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.Spezifikation
AAH69540.1 | |
20 μg | |
Ser 23 - Phe 202 | |
LIF,CDF,DIA,HILDA,MLPLI | |
Unconjugated | |
Tag Free | |
Less than 1.0 EU / μg by the LAL method. | |
Powder | |
>95% as determined by SDS-PAGE |
19.9 kDa | |
ActiveMax™ Human LIF, Tag Free (LIF-H521b) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Phe 202 (Accession # AAH69540.1). | |
-20°C | |
LIF | |
Human | |
PBS, pH7.4 | |
HEK293 | |
ActiveMax™ Cytokines |