beta-Amyloid Monoclonal Antibody, Clone: 252Q6, Invitrogen™

Beschreibung

beta-Amyloid Antibody (252Q6), AMB0062, from Invitrogen™. Species Reactivity: Mouse, Rat; Applications: ELISA, Western Blot.

Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer's disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE). This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. Found associated with the presenilins is the transmembrane glycoprotein nicastrin. Nicastrin has been found to bind to the carboxyl-terminus of betaAPP and helps to modulate the production of the amyloid beta peptide.
Host Species: Mouse
Cross Reactivity: Mouse, Rat
Isotype: IgG1, κ
Clone: 252Q6
Immunogen: A chemically synthesized peptide derived from amino acid residues 1-20 of mouse A-beta. This region is conserved in rat A-beta.
Formulation: 100μg purified antibody in PBS with 1% BSA and 0.1% sodium azide; pH 7.2
Synonyms: AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN2, Alzheimer disease amyloid A4 protein homolog, APP, Amyloidogenic glycoprotein AG, Amyloid beta A4 protein, amyloid beta (A4) precursor protein, Peptidase nexin-II
Uniprot Accession: P08592, P12023
Entrez GeneID: 11820, 54226
Gene Symbol: APP
This product detects beta-Amyloid in Mouse and Rat samples. This product has been successfully used in ELISA and Western Blot procedures. The immunogen is A chemically synthesized peptide derived from amino acid residues 1-20 of mouse A-beta. This region is conserved in rat A-beta..
References:
Test: ELISA, Species: Ms, Reference: Emergence of a seizure phenotype in aged apolipoprotein epsilon 4 targeted replacement mice. (Jul 2012) Brain Res., Vol: 1467, , 120-32, Hunter JM,Cirrito JR,Restivo JL,Kinley RD,Sullivan PM,Holtzman DM,Koger D,Delong C,Lin S,Zhao L,Liu F,Bales K,Paul SM; Pubmed ID: 22682924