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CD19 Monoclonal Antibody (eBio1D3 (1D3)), Super Bright™ 702, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
163.00 € - 351.00 €
Spezifikation
Antigen | CD19 |
---|---|
Klon | eBio1D3 (1D3) |
Konzentration | 0.2 mg/mL |
Anwendungen | Flow Cytometry |
Klassifikation | Monoclonal |
Produktcode | Marke | Menge | Preis | Menge & Verfügbarkeit | |||||
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Produktcode | Marke | Menge | Preis | Menge & Verfügbarkeit | |||||
15903714
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Affymetrix eBioscience
67-0193-80 |
25 μg |
163.00 €
25 Mikrogramm |
Verfügbar ab: 14-05-2024 Loggen Sie sich ein, um den Lagerbestand zu sehen |
Bitte melden Sie sich an, um diesen Artikel zu kaufen. Sie benötigen ein Web-Konto? Registrieren Sie sich noch heute! | ||||
15913714
|
Affymetrix eBioscience
67-0193-82 |
100 μg |
351.00 €
100 Mikrogramm |
Verfügbar ab: 14-05-2024 Loggen Sie sich ein, um den Lagerbestand zu sehen |
Bitte melden Sie sich an, um diesen Artikel zu kaufen. Sie benötigen ein Web-Konto? Registrieren Sie sich noch heute! | ||||
Beschreibung
Description: The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.
Applications Reported: This eBio1D3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio1D3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 702 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.Spezifikation
CD19 | |
0.2 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
PBS with BSA and 0.09% sodium azide; pH 7.2 | |
AW495831; B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Cd19; CD19 antigen; CD19 molecule; CVID3; differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12 | |
Cd19 | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
Cd19 |
eBio1D3 (1D3) | |
Flow Cytometry | |
Super Bright 702 | |
Rat | |
Mouse | |
P25918 | |
12478 | |
IgG2a κ | |
Affinity chromatography | |
Antibody |