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Human CDK7/Cyclin H/MNAT1, His Tag Recombinant Protein
Human CDK7/Cyclin H/MNAT1, His Tag Recombinant Protein
Marke: Invitrogen PV4186
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Beschreibung
The trimeric complex of CDK7, cyclin H and MNAT1, functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This complex is thought to serve as a direct link between the regulation of transcription and the cell cycle.
Spezifikation
| NP_001790, Q130R, NP_001230, NP_002422 | |
| Protein Assays and Analysis, Kinase Assay | |
| 126.3kDa | |
| 1 mg | |
| For research use only. Not for use in diagnostic procedures | |
| CDK7/Cyclin H/MNAT1 Recombinant Human Protein | |
| CDK and CHK | |
| 96nmoles of phosphate transferred to CDK7/9tide peptide substrate (YSPTSPSYSPTSPSYSPTSPSKKKK) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 3.33μg/mL | |
| His Tag | |
| Frozen |
| 0.4mg/mL | |
| Frozen | |
| CDK7/Cyclin H/MNAT1 Recombinant Human Protein | |
| –80°C | |
| CDK7/Cyclin H/MNAT1 | |
| CDK7/cyclin H/MNAT1 was pre-diluted in enzyme dilution buffer and assayed in 12.5mM Tris (pH 7.5), 10mM MgCl2, 1mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 200μM ATP, 333μg/mL CDK7/9tide peptide substrate (YSPTSPSYSPTSPSYSPTSPSKKKK) and trace [32P]-γ-ATP for 10 minutes at 30°C | |
| CDK7/cyclin H/MNAT1 Recombinant Human Protein | |
| Human | |
| Baculovirus | |
| 70% by SDS-PAGE |
For Research Use Only. Not for use in diagnostic procedures.