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STEMCELL Technologies ClonaCell™-CHO CD Medium
Chemically defined, animal component-free, serum-free, protein-free, glutamine-free semi-solid methylcellulose-based medium for selection and cloning of CHO cells
Marke: STEMCELL Technologies 03815
Weitere Details : Netto-Gewicht : 0.10000kg
Beschreibung
- ClonaCell™-CHO CD Medium is a methylcellulose-based semi-solid medium recommended for selection and cloning of Chinese hamster ovary (CHO) cells
- This medium is chemically defined, protein-free, and animal component-free
- It does not contain L-glutamine, selection agents, or phenol red and is compatible with dihydrofolate reductase (DHFR) and glutamine synthetase (GS) selection systems
- Benefits of semi-solid cloning: Individual clones and their progeny remain localized together in a semi-solid matrix as they grow to form distinct monoclonal colonies
- This prevents the loss of rare, high-producing clones by overgrowth from faster-growing cells, as can occur during selection in a liquid medium, and facilitates the isolation of a diverse set of clones with a wide range of growth rates and productivities to be obtained for downstream screening
- Colonies obtained from semi-solid medium have a high probability of monoclonality, allowing clonal cell lines to be generated in less time and using fewer resources than with limiting dilution cloning
- Colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid growth medium for screening and expansion
Spezifikation
| CHO | |
| Cell Culture; Semi-Solid Cloning | |
| 90 mL |