Invitrogen™ FAP Human ELISA Kit

Beschreibung

The Human FAP ELISA quantitates Hu FAP in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FAP. Principle of the method The Hu FAP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Das von diesem Gen codierte Protein ist eine homodimerische integrale Membran-Gelatinase aus der Serinprotease-Familie. Es wird selektiv in reaktiven Stromafibroblasten von Epithelkarzinomen, in Granulationsgewebe von heilenden Wunden und in malignen Zellen von Knochen- und Weichgewebssarkomen exprimiert. Es wird angenommen, dass dieses Protein an der Steuerung des Fibroblastenwachstums oder der epithelial-mesenchymalen Transition während der Entwicklung, Gewebereparatur und epithelialen Karzinogenese beteiligt ist.