Invitrogen™ Human MMP-15 Recombinant Protein

Beschreibung

MMP15 Recombinant Protein, RP-77534, from Invitrogen.

RP-77534 is a purified recombinant MMP-15 (catalytic domain) protein purified from E. coli periplasm. MT2-MMP catalytic domain is produced by activation of a recombinant soluble proform of MT2-MMP. The sequence spans Tyr91-Gly267 of mature human MT2-MMP. The catalytic domain consists of 177 amino acids with a calculated MW of 20kDa. This domain represents more than 95% of the protein. The purity is 95% by SDS-PAGE, and concentration measured using a BCA-Assay. The specific activity >30mU/mg, where 1U hydrolyzes 1μmol peptide within 1 minute. The catalytic domain is inhibited by tissue inhibitors of matrix metalloproteinases 2 and 3 (TIMP-2 and TIMP-3) and by chelators of divalent cations like EDTA or o-phenanthroline. This product can be used to study the activation of TIMP-2 and the degradation of proteins of the extracellular matrix. The enzyme allows screening of TIMP inhibitors and characterization of inhibitor action.