Invitrogen™ PGI Human ELISA Kit

Beschreibung

The Human Pepsinogen I (Hu PGI) ELISA quantitates Hu PGI in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PGI. Principle of the method The Human PGI solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Pepsinogen ist die inaktive Vorläuferform von Pepsin, einem der wichtigsten proteolytischen Enzyme, die von der Magenschleimhaut abgesondert werden. Pepsinogen wird in der Magenschleimhaut von Hauptzellen synthetisiert. Nach der Sekretion wird Pepsinogen in Gegenwart von HCl aktiviert, wodurch eine saure Umgebung im Magen entsteht, wodurch sich Pepsinogen autokatalytisch entfalten und spalten kann. Pepsin besteht aus einer einzelnen Polypeptidkette und entsteht aus seinem Vorläufer, Pepsinogen, durch Entfernung eines 41-Aminosäuresegments aus dem N-Terminus.