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IgM Monoclonal Antibody (SA-DA4), Super Bright™ 600, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Marke: Affymetrix eBioscience 63-9998-42
Weitere Details : Netto-Gewicht : 0.01000kg
Beschreibung
Description: The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells. Applications Reported: This SA-DA4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This SA-DA4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10<sup>5</sup> to 10<sup>8</sup> cells/test. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No. SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation.
IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.Spezifikation
IgM | |
Monoclonal | |
5 μL/Test | |
PBS with BSA and 0.09% sodium azide; pH 7.2 | |
P01871 | |
IGHM | |
Affinity chromatography | |
RUO | |
3507 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
SA-DA4 | |
Super Bright 600 | |
IGHM | |
AGM1; IGHM; immunoglobulin heavy constant mu; MU; VH | |
Mouse | |
100 Tests | |
Primary | |
Human | |
Antibody | |
IgG1 κ |
For Research Use Only