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IL-1 beta Armenian Hamster anti-Mouse, Rat, Clone: B122, eBioscience™

Armenian Hamster Monoclonal Antibody

Marke:  Affymetrix eBioscience 14-7012-81

Weitere Details : Netto-Gewicht : 0.01000kg

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Artikelnummer. 15257977

  • 96.50 € / 50 Mikrogramm

Verfügbar ab
26-04-2021

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Beschreibung

Beschreibung

Description: The B122 antibody reacts with mouse and rat interleukin-1beta (IL-1beta), a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. Applications Reported: The B122 antibody has been reported for use in cytokine neutralization, immunoblotting (WB), and ELISA. Please refer to Functional Grade purified B122 (cat. 16-7012) for functional assays. Applications Tested: The B122 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-1 beta (IL-1 beta) in combination with the biotinylated polyclonal anti-mouse interleukin-1 beta for detection and recombit mouse IL-1 beta (14-8012) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombit standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.
Spezifikation

Spezifikation

IL-1 beta
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P10749, Q63264
Interleukin-1 beta, Catabolin, IL1B
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse, Rat
ELISA, Functional Assay, Neutralization
B122
Unconjugated
Il1b
Liquid
Il1b
IgG
50 μg
4° C
Primary
16176, 24494
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

For Research Use Only.