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Invitrogen™ M-CSF (CSF1) Mouse ELISA Kit
Sandwich ELISA Kit
628.00 € - 2635.00 €
Spezifikation
Zugriffsnummer | Gehäuse P07141 |
---|---|
Assay-Empfindlichkeit: | 1 pg/mL |
Assay | Festphasen-Sandwich-ELISA |
Konjugat | Biotin |
Produkttyp | ELISA-Kit |
Produktcode | Marke | Menge | Preis | Menge & Verfügbarkeit | |||||
---|---|---|---|---|---|---|---|---|---|
Produktcode | Marke | Menge | Preis | Menge & Verfügbarkeit | |||||
15435243
|
Invitrogen™
EMCSF1 |
96 Test-Kit |
628.00 €
1 Set |
Verfügbar ab: 23-05-2024 Loggen Sie sich ein, um den Lagerbestand zu sehen |
Bitte melden Sie sich an, um diesen Artikel zu kaufen. Sie benötigen ein Web-Konto? Registrieren Sie sich noch heute! | ||||
17185699
|
Invitrogen™
EMCSF1X5 |
5 x 96 Tests |
2635.00 €
1 Stück |
Verfügbar ab: 31-05-2024 Loggen Sie sich ein, um den Lagerbestand zu sehen |
Bitte melden Sie sich an, um diesen Artikel zu kaufen. Sie benötigen ein Web-Konto? Registrieren Sie sich noch heute! | ||||
Beschreibung
The Mouse M-CSF (CSF1) ELISA quantitates Ms M-CSF in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms M-CSF. Principle of the method The Mouse M-CSF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.Spezifikation
Gehäuse P07141 | |
Festphasen-Sandwich-ELISA | |
ELISA-Kit | |
Plasma, Serum, Überstand | |
Maus | |
Kolorimetrisches Mikrotiterplatten-Lesegerät | |
C87615, CSFM, MCSF, op | |
< 10 % | |
RUO | |
2 bis 8 °C | |
Maus | |
4 Std 45 Min |
1 pg/mL | |
Biotin | |
Wachstumsfaktoren und Rezeptoren | |
Dieses ELISA-Kit zeigt keine Kreuzreaktivität mit den folgenden getesteten Zytokinen: Maus CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fraktalkin, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN (OB), LIX, L-Selektin, Lymphotactin, MCP-1, MCP-5, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selektin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. | |
ELISA, Protein-Assays, Proteinbiologie, Krebsbiologie, Immunologie, Neurowissenschaften, Neurobiologie, Proteinnachweis, Proteinanalyse | |
12977 | |
< 12 % | |
HRP | |
Entwicklung | |
C87615, Csfm, MCSF, op | |
1 Std 20 Min |