Mouse IgG2a Isotype Control, APC, Invitrogen™

Beschreibung

Mouse IgG2a Isotype Control, APC conjugate, MA1-10420, from Invitrogen.

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody’s species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Host Species: Mouse
Isotype: IgG2a
Clone: PPV-04
Immunogen: Plum pox virus
Formulation: 100 ug purified isotype control antibody in PBS with 0.2% BSA and 15mM sodium azide
Synonyms: Negative Control, Immunoglobulin Control, Control
MA1-10420 can be used as a negative control to measure the non-specific binding of Mouse IgG2a in human, porcine, mouse and rat tissues.