Invitrogen™ Prolactin (PRL) Mouse ELISA Kit

Beschreibung

The Mouse Prolactin (PRL) ELISA quantitates Ms PRL in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms PRL. Principle of the method The Mouse PRL solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Prolactin (PRL) oder Luteotropes Hormon (LTH) ist ein Peptidhormon, das von Dr. Henry Friesen entdeckt wurde und hauptsächlich mit der Laktation assoziiert ist. Beim Stillen stimuliert das Saugen des Säuglings die Produktion von Prolaktin, das die Brust in Vorbereitung auf das nächste Füttern mit Milch über einen Prozess namens Laktogenese füllt. Oxytocin, ein weiteres Hormon, wird ebenfalls freigesetzt, wodurch Milchablass ausgelöst wird.