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CD138 Rat anti-Mouse, APC-R700, Clone: 281-2, BD
Rat Monoclonal Antibody
Marke: BD Biosciences 565176
Weitere Details : Netto-Gewicht : 0.01000kg
Beschreibung
The 281-2 monoclonal antibody specifically binds to the core protein of CD138 (Syndecan-1), a cell-surface, integral membrane heparan sulfate- and chondroitin sulfate-containing proteoglycan that binds to interstitial extracellular matrix molecules. Syndecan-1 is predominantly expressed on epithelial cells, where its expression correlates with normal epithelial organization. It is also expressed on B lymphocytes at specific stages during their differentiation: precursor B cells in the bone marrow and antibody-secreting cells, including plasma cells, but not mature peripheral B cells. It is thus implicated in mediating B cell-matrix interactions. CD138 expression is also regulated during embryonic development, and the molecule shows a tissue- specific structural polymorphism resulting from different post-translational modifications. The 281-2 antibody may be used to detect the differently glycosylated forms, because it reacts with the core protein. Furthermore, the mAb detects the Syndecan-1 ectodomain which is cleaved from cell surfaces by a metalloproteinase.
This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor™ 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor™ 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor™ (eg, 730/45-nm filter).
Spezifikation
CD138 | |
Monoclonal | |
0.2mg/mL | |
SYND1; Syndecan-1; syn-1; Sdc1; Sstn; synstatin | |
Rat | |
Affinity Purified | |
RUO | |
Mouse | |
IgG2a κ |
Flow Cytometry | |
281-2 | |
APC-R700 | |
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide. | |
NAMRU mouse mammary gland epithelial cell line NMuMG | |
50 μg | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |