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IL1B Recombinant Human Protein, Invitrogen™ Sino Biological™
This recombinant protein was expressed from a DNA sequence encoding the mature form of human IL1B (NP_000567.1) (Ala 117-Ser 269)
Brand: Invitrogen 10139HNAE25
Additional Details : Netto-Gewicht : 0.10000kg
Description
Interleukin 1, beta (IL1B), a recombinant human protein, is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as immunogens, protein standards, and in other cell biology research applications.- Activity: 1.Measured by its binding ability in a functional ELISA; a. Immobilized recombinant human IL1B at 2μg/mL (100μL/well) can bind recombinant human IL1R2 with a linear range of 6.25-400ng/mL; b. Immobilized human IL1B at 10μg/mL (100μL/well) can bind human IL1R1, the EC50 of human IL1R1 is 0.48μg/mL; 2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells, the ED50 for this effect is typically 0.5-5pg/mL
- Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
This protein is manufactured by Sino Biological Inc.
Specifications
| Protein Expression and Isolation, Enzyme and Protein Activity Assay, Protein Assay Control, Reference Standards and Accessories | |
| 17kDa | |
| Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed. | |
| Interleukin 1, beta | |
| Untagged | |
| Cytokines & Receptors | |
| ≥90% by SDS-PAGE |
| 3553 | |
| 5 x 5μg | |
| IL-1, IL1-BETA, IL1F2, IL1B | |
| IL1B | |
| E. coli | |
| Lyophilized |
For Research Use Only. Not for use in diagnostic procedures.