Reagenzien für die Proteinmodifikation

Thermo Scientific™ Pierce™ Proteasen für Massenspektrometer

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations. GLU-C 50µG

DTT, 1M

DTT, 1M 1M DDT (Dithiothreitol), 1 ml

Thermo Scientific™ Pierce™ DTT (Dithiothreitol)

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent. DTT 5GM

Thermo Scientific™ Pierce™ Methyl-PEG-NHS-Ester-Reagenzien

Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units). MS(PEG)12, 1 g, 1g

Thermo Scientific™ Pierce™ immobilisiertes TCEP-Disulfid-Verringerungsgel

Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized. Immobilized TCEP Disulfide Reducing Gel 5 mL

Thermo Scientific™ Pierce™ Methyl-PEG-Maleimide Reagents

Pegylate sulfhydryls (-SH groups) on proteins (cysteine residues) or assay surfaces using MS(PEG); sulfhydryl-reactive (maleimide), PEG compounds (12 and 24 EG units). MM(PEG)12, 1 g, 1 g

Molecular Probes™ Dithiothreitol (DTT)

Dithiothreitol (DTT) Dithiothreitol (DTT), 1 g

Thermo Scientific™ Pierce™ Humanes Rhinovirus 3C Protease (HRV 3C)

Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage. 10KU Human Rhinovirus 3C Protease

Thermo Scientific™ Pierce™ N-Ethylmaleimide (NEM)

Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation. NEM OR N-ETHYLMALEIMIDE 25GM

Thermo Scientific™ Pierce™ Kit für tryptischen In-Gel-Verdau

Trypsin-digest and recover peptide fragments for mass spectrometry from 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs). In-Gel Tryptic Digestion Kit

Thermo Scientific™ Pierce™ In-Solution Tryptic Digestion and Guanidination Kit

Alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry (MS) using proteomics-grade trypsin, buffers and reagents. In-Solution Tryptic Digestoin & Guanidination KitKIT

Thermo Scientific™ Pierce™ TCEP·HCl

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3). TCEP*HCl, 1 gm

Thermo Scientific™ Pierce™ Iodessigsäure

Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide. IODOACETIC ACID 500MG

Thermo Scientific™ WELQut Protease

Thermo Scientific™ WELQut Protease is a highly specific serine protease cleaving outside the WELQX recognition motif to remove N-terminal protein fusion tags. WELQut Protease

Thermo Scientific™ Pierce™ TPCK Trypsin und Harz

Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion. Immobilized Trypsin, TPCK Treated, 2 mL

Thermo Scientific™ Pierce™ Traut' s Reagenz

2-Iminothiolane-HCl (CAS 4781-83-3); an amine-reactive thiolation reagent to cap protein primary amines with sulfhydryl groups for immediate crosslinking. 2-Iminothiolane-HCl (Traut's Reagent) 500 mg

Thermo Scientific™ Pierce™ SATA

Short (2.8A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies. SATA, 50 mg

Thermo Scientific™ Pierce™ Proteinase K

Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease. PROTEINASE K 100MG

Thermo Scientific™ Pierce™ 8M Guanidine·HCl-Lösung

Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods. 8M Guanidine-HCl, 200 ml

Thermo Scientific™ Pierce™ Premium Grade TCEP-HCl

Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this disulfide reducing agent. 100GR PREMIUM GRADE TCEP

Thermo Scientific™ Pierce™ immobilisiertes Pepsin

Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin. Immobilized Pepsin - Agarose, 5 mL

Alfa Aesar™ Heterogeneous Catalyst Kit, 40 sample

Heterogeneous Catalyst Kit, 40 sample 1each

Thermo Scientific™ Pierce™ Cysteine·HCl

Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl) CAS 52-89-1. CYSTEINE HCL 5GM

Thermo Scientific™ Pierce™ Carboxy-PEG-Amine Compounds

CA(PEG)n: pegylated amino acids of the form carboxy-PEG-amine; with 4 to 24 polyethylene glycol units; for surface- and molecule-pegylation applications. CA(PEG)12, 1 g, 1g

Thermo Scientific™ Pierce™ Methyl-PEG-Amine Compounds

MA(PEG)n: methyl- and amine-capped polyethylene glycol compounds containing 4 to 24 PEG units; use for surface-modification and molecule-pegylation applications. MA(PEG)24,1 g, 1g

Thermo Scientific™ Pierce™ immobilisiertes Ficin

Enable controlled antibody fragmentation, especially of mouse IgG1, with this sulfhydryl-specific ficin protease immobilized onto beaded agarose resin. Immobilized Ficin, 5 mL

Thermo Scientific™ Pierce™ Staphylococcus aureus V-8 Protease and Kt

Specifically cleave only at glutamic acid residues or cleave at both glutamic and aspartic residues with our immobilized Staphylococcus aureus V-8 protease kit. Immobilized V-8 Protease-Kit

Thermo Scientific™ Pierce™ Hydroxylamin·HCl

NH2OH (CAS 7803-49-8) to cleave Asn-Gly peptide bonds or deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent. HYDROXYLAMINE-HCL 25GM

Thermo Scientific™ Pierce™ MMTS

Methyl methanethiosulfonate (CAS 2949-92-0); reversibly blocks cysteines (sulfhydryl groups) to facilitate study of enzyme activation and protein functions. MMTS 200MG

Thermo Scientific™ Pierce™ Sulfhydryl Addition Kit

Modify protein primary amines to create free sulfhydryl groups for labeling, crosslinking or immobilization techniques; complete kit with SATA reagent and hydroxylamine. Sulfhydryl Addition kit

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