Reagenzien für die Proteinmodifikation

Thermo Scientific™ Pierce™ Staphylococcus aureus V-8 Protease and Kt

Specifically cleave only at glutamic acid residues or cleave at both glutamic and aspartic residues with our immobilized Staphylococcus aureus V-8 protease kit. Immobilized V-8 Protease-Kit

Thermo Scientific™ Pierce™ Guanidine·HCl

Pure, crystalline guanidium hydrochloride (GdnHCl, GuHCl), CAS 50-01-1; denaturant for protein biology methods (elution, solubilization, refolding, storage). GUANIDINE-HCL 500GM

Thermo Scientific™ Pierce™ 2-Mercaptoethanol

Pure, liquid (equals 14M), beta-mercaptoethanol (bME, 2BME, 2-ME, b-mer), CAS 60-24-2; thiol reducing agent for cleaving protein disulfide bonds (cystines cysteines). 2-MERCAPTOETHANOL 10ML

Thermo Scientific™ Pierce™ Proteasen für Massenspektrometer

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations. CHYMOTRYPSIN 100µG

Thermo Scientific Pierce™ Premium Grade TCEP-HCl

Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this disulfide reducing agent. 100GR PREMIUM GRADE TCEP

Thermo Scientific™ Pierce™ DTT (Dithiothreitol)

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent. X48 NO-WEIGH DTT

Thermo Scientific™ Pierce™ Sulfo-NHS-Acetate

Amine-reactive (NHS ester) compound to permanently block primary amines (e.g., protein lysine side chains) to prevent interactions or later conjugation. Sulfo-NHS-Acetate, 100mg, 100 mg

Thermo Scientific™ Bond-Breaker™ pH-neutrale TCEP-Lösung

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers. Bond-Breaker TCEP Solution, Neutral pH 5 mL

Thermo Scientific™ Pierce™ TPCK Trypsin und Harz

Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion. Immobilized Trypsin, TPCK Treated, 2 mL

Thermo Scientific™ Pierce™ immobilisiertes Papain

Generate and purify Fab fragments from antibodies with cysteine-endopeptidase papain immobilized onto beaded agarose resin. Immobilized Papain - Agarose, 5 mL

Thermo Scientific™ Pierce™ immobilisiertes TCEP-Disulfid-Verringerungsgel

Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized. Immobilized TCEP Disulfide Reducing Gel 5 mL

Thermo Scientific™ Pierce™ Humanes Rhinovirus 3C Protease (HRV 3C)

Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage. 10KU Human Rhinovirus 3C Protease

Thermo Scientific™ Pierce™ Methyl-PEG-Maleimide Reagents

Pegylate sulfhydryls (-SH groups) on proteins (cysteine residues) or assay surfaces using MS(PEG); sulfhydryl-reactive (maleimide), PEG compounds (12 and 24 EG units). MM(PEG)24 100MG

Thermo Scientific™ Pierce™ TCEP·HCl

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3). TCEP·HCL 10GM

Thermo Scientific™ Pierce™ In-Solution Tryptic Digestion and Guanidination Kit

Alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry (MS) using proteomics-grade trypsin, buffers and reagents. In-Solution Tryptic Digestoin & Guanidination KitKIT

Thermo Scientific™ Pierce™ Carboxypeptidase Y and Resin

Cleave (release) terminal amino acids including proline from the C-terminus of peptides using this protease; its immobilized form allows enzyme removal after treatment. IMMOB CARBOXYPEPTIDASE Y 2ML

Thermo Scientific™ Pierce™ TFCS

Modify proteins by adding a 7-atom spacer arm with this amine-reactive (NHS ester) compound; the terminal trifluoroacetyl group can be removed to expose a new primary amine. TFCS 50MG

Thermo Scientific™ Pierce™ Methyl-PEG-Amine Compounds

MA(PEG)n: methyl- and amine-capped polyethylene glycol compounds containing 4 to 24 PEG units; use for surface-modification and molecule-pegylation applications. MA(PEG)12,1 g, 1g

Thermo Scientific™ Pierce™ Carboxy-PEG-Amine Compounds

CA(PEG)n: pegylated amino acids of the form carboxy-PEG-amine; with 4 to 24 polyethylene glycol units; for surface- and molecule-pegylation applications. CA(PEG)24, 1 g, 1g

Thermo Scientific™ Pierce™ SATP

Short (4.1A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies. SATP, 50 mg

Thermo Scientific™ Pierce™ Natriummetaperiodat, Kristallin-ACS-Grad

Gently oxidize glycoprotein carbohydrate sugars to create reactive aldehydes (carbonyls) for crosslinking and immobilization using NaIO4 (CAS 7790-28-5). SODIUM META-PERIODATE 25GM

Thermo Scientific™ Pierce™ Immobilized Reductant Columns

Efficiently reduce protein or peptide disulfide bonds with this reusable column on with an immobilized thiol-based reducing agent for solid-phase disulfide bond reduction. REDUCE-IMM IMMOB REDUCTANT COL2ML

Thermo Scientific™ Pierce™ MMTS

Methyl methanethiosulfonate (CAS 2949-92-0); reversibly blocks cysteines (sulfhydryl groups) to facilitate study of enzyme activation and protein functions. MMTS 200MG

Thermo Scientific™ Pierce™ Hydroxylamin·HCl

NH2OH (CAS 7803-49-8) to cleave Asn-Gly peptide bonds or deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent. HYDROXYLAMINE-HCL 25GM

Thermo Scientific™ Pierce™ Methyl-PEG-NHS-Ester-Reagenzien

Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units). 100MG METHYL-PEO8-NHS ESTER

Thermo Scientific™ Pierce™ 2-Mercaptoethylamine·HCl

Pure, crystalline 2-aminoethanethiol (2-MEA-HCl, also called cysteamine-HCl), CAS 156-57-0; selectively reduces antibody hinge-region disulfide bonds. 2-MEA-HCL 36MG

Thermo Scientific™ Pierce™ Carboxy-PEG-Lipoamide Compounds

CL(PEG)n: carboxylic acid and bidentate thiol compound with a 4-unit polyethylene glycol (PEG) spacer; carboxylate and pegylate quantum dots, gold surfaces, silver. CL(PEG)12, 100MG

Thermo Scientific™ Pierce™ Cysteine·HCl

Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl) CAS 52-89-1. CYSTEINE HCL 5GM

Thermo Scientific™ Pierce™ SATA

Short (2.8A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies. SATA, 50 mg

Thermo Scientific™ Pierce™ Factor Xa

Protease (serine endopeptidase for prothrombin); cleaves arginine in the sequence Ile-Glu-Gly-Arg; useful for His-tagged fusion protein engineering. FACTOR XA(BOVINE)50µG

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