Reagenzien für die Proteinmodifikation

Thermo Scientific™ Pierce™ Carboxy-PEG-Lipoamide Compounds

CL(PEG)n: carboxylic acid and bidentate thiol compound with a 4-unit polyethylene glycol (PEG) spacer; carboxylate and pegylate quantum dots, gold surfaces, silver. CL(PEG)12, 100MG

Thermo Scientific™ Pierce™ Carboxy-PEG-Amine Compounds

CA(PEG)n: pegylated amino acids of the form carboxy-PEG-amine; with 4 to 24 polyethylene glycol units; for surface- and molecule-pegylation applications. CA(PEG)8 100MG

Thermo Scientific™ Pierce™ Proteasen für Massenspektrometer

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations. LYS-C 20µG

Thermo Scientific™ Pierce™ AEDP Crosslinker

Crosslink amines to carboxyls with this spacer containing an internal disulfide bond; use EDC to modify functional groups on proteins and surfaces. AEDP 50MG

Thermo Scientific™ Pierce™ immobilisiertes TCEP-Disulfid-Verringerungsgel

Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized. Immobilized TCEP Disulfide Reducing Gel 5 mL

Thermo Scientific™ Pierce™ SAT(PEG)4

Long (18.2A), pegylated form of SATA, an amine-reactive (NHS ester) sulfhydryl-addition reagent; contains 4-unit polyethylene glycol spacer arm. PEO4-SATA, 100 mg

Thermo Scientific™ Pierce™ Hydroxylamin·HCl

NH2OH (CAS 7803-49-8) to cleave Asn-Gly peptide bonds or deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent. HYDROXYLAMINE-HCL 25GM

Thermo Scientific™ Pierce™ Methyl-PEG-NHS-Ester-Reagenzien

Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units). 100MG METHYL-PEO8-NHS ESTER

Thermo Scientific™ Pierce™ N-Ethylmaleimide (NEM)

Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation. NEM OR N-ETHYLMALEIMIDE 25GM

Thermo Scientific™ Pierce™ EMCA

Permanently cap protein or peptide cysteines with carboxyl groups (make caproic acid derivatives) using this sulfhydryl-reactive (maleimide) compound. EMCA, 1 gm

Thermo Scientific™ Pierce™ Methyl-PEG-Lipoamide Compounds

ML(PEG)n: methyl and bidentate thiol compound with a 4-unit polyethylene glycol (PEG) spacer; methylate and pegylate quantum dots, gold surfaces, silver. ML(PEG)4, 100mg

Thermo Scientific™ GlycoLink™ Coupling Catalyst

Enhance aldehyde-hydrazide crosslinking efficiency for glycoprotein immobilization and conjugation with analine (CAS 62-53-3) and an optimized buffer system. 100ML GLYCOLINK COUPLING CATALYST

Thermo Scientific™ Pierce™ Methyl-PEG-Amine Compounds

MA(PEG)n: methyl- and amine-capped polyethylene glycol compounds containing 4 to 24 PEG units; use for surface-modification and molecule-pegylation applications. MA(PEG)4 100MG

Thermo Scientific Pierce™ Premium Grade TCEP-HCl

Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this disulfide reducing agent. 10GR PREMIUM GRADE TCEP

Thermo Scientific™ Pierce™ TCEP·HCl

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3). TCEP*HCl, 1 gm

Thermo Scientific™ Pierce™ Iodessigsäure

Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide. IODOACETAMIDE, SINGLE-USE

Thermo Scientific™ Pierce™ immobilisiertes Papain

Generate and purify Fab fragments from antibodies with cysteine-endopeptidase papain immobilized onto beaded agarose resin. Immobilized Papain - Agarose, 5 mL

Thermo Scientific™ Pierce™ TPCK Trypsin und Harz

Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion. TPCK TRYPSIN 50MG

Thermo Scientific™ Pierce™ Factor Xa

Protease (serine endopeptidase for prothrombin); cleaves arginine in the sequence Ile-Glu-Gly-Arg; useful for His-tagged fusion protein engineering. FACTOR XA(BOVINE)250µG

Thermo Scientific™ Pierce™ Immobilized Reductant Columns

Efficiently reduce protein or peptide disulfide bonds with this reusable column on with an immobilized thiol-based reducing agent for solid-phase disulfide bond reduction. REDUCE-IMM IMMOB REDUCTANT COL2ML

Thermo Scientific™ Pierce™ Guanidine·HCl

Pure, crystalline guanidium hydrochloride (GdnHCl, GuHCl), CAS 50-01-1; denaturant for protein biology methods (elution, solubilization, refolding, storage). GUANIDINE-HCL 500GM

Thermo Scientific™ Pierce™ TFCS

Modify proteins by adding a 7-atom spacer arm with this amine-reactive (NHS ester) compound; the terminal trifluoroacetyl group can be removed to expose a new primary amine. TFCS 50MG

Thermo Scientific™ Pierce™ Traut' s Reagenz

2-Iminothiolane-HCl (CAS 4781-83-3); an amine-reactive thiolation reagent to cap protein primary amines with sulfhydryl groups for immediate crosslinking. 2-Iminothiolane-HCl (Traut's Reagent) 500 mg

Thermo Scientific™ Pierce™ Citraconic Anhydride

Reversibly cap lysines and other primary amines with carboxyl groups using this amine-reactive compound; stable at pH 7 but hydrolyzes (decouples) at pH 3 to 4 (CAS 616-02-4). CITRACONIC ANHYDRIDE 100GM

Thermo Scientific™ Pierce™ Kit für tryptischen In-Gel-Verdau

Trypsin-digest and recover peptide fragments for mass spectrometry from 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs). In-Gel Tryptic Digestion Kit

Thermo Scientific™ Pierce™ DTT (Dithiothreitol)

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent. X48 NO-WEIGH DTT

Thermo Scientific™ Pierce™ Staphylococcus aureus V-8 Protease and Kt

Specifically cleave only at glutamic acid residues or cleave at both glutamic and aspartic residues with our immobilized Staphylococcus aureus V-8 protease kit. S. AUREUS PROTEASEV-8(2500U)5MG

Thermo Scientific™ Pierce™ Proteinase K

Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease. PROTEINASE K 100MG

Thermo Scientific™ Pierce™ MMTS

Methyl methanethiosulfonate (CAS 2949-92-0); reversibly blocks cysteines (sulfhydryl groups) to facilitate study of enzyme activation and protein functions. MMTS 200MG

Molecular Probes™ Dithiothreitol (DTT)

Dithiothreitol (DTT) Dithiothreitol (DTT), 1 g