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Invitrogen™ IGFBP6 Human ELISA Kit
Sandwich ELISA Kit
Marke: Invitrogen™ EHIGFBP6
Weitere Details : Netto-Gewicht : 0.00400kg
Beschreibung
The Human Insulin-like Growth Factor Binding Protein 6 (Hu IGFBP6) ELISA quantitates Hu IGFBP6 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IGFBP6. Principle of the method The Human IGFBP6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
IGFBP6 kodiert für ein216-Aminosäureprotein mit einem berechneten Molekulargewicht von 22,847 kDa. Das Protein wurde als Autocrin-Wachstumshemmer erkannt. Rekombinantes IGFBP6 hemmt nachweislich das Wachstum von HaCat-Zellen und anderen keratinozyten Zelllinien.Spezifikation
| Gehäuse P24592 | |
| 150 pg/mL | |
| Festphasen-Sandwich-ELISA | |
| ELISA-Kit | |
| Plasma, Serum, Überstand | |
| 96 Assays | |
| ELISA, Protein-Assays, Proteinbiologie, Krebsbiologie, Immunologie, Neurowissenschaften, Neurobiologie, Proteinnachweis, Proteinanalyse | |
| 3489 | |
| < 12 % | |
| Vorbeschichtete 96-Well-Platte, Assay-Verdünnungskonzentrat, Biotinylierter Nachweisantikörper, Waschpuffer, Stopplösung, Dichtungsfolien für Mikrotiterplatten, Chromogen, Stopplösung, Dichtungsfolien für Mikrotiterplatten | |
| 96 Test-Kit | |
| Signaltransduktion | |
| 2 bis 8 °C | |
| Human | |
| 4 Std 45 Min |
| 82.3 bis 60,000 pg/ml | |
| 150 bis 60000 pg/ml | |
| Biotin | |
| Weitere Proteine | |
| Dieses ELISA-Kit weist keine Kreuzreaktivität mit einem der getesteten Zytokine auf: Humanes Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. | |
| Human | |
| Kolorimetrisches Mikrotiterplatten-Lesegerät | |
| Gehäuse IBP6 | |
| < 10 % | |
| HRP | |
| RUO | |
| Plasma, 5 μl; Serum, 5 μl; Überstand, 100 μl | |
| Gehäuse IBP6 | |
| 1 Std 20 Min |